Category: 5. Biology
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Detection of mosaic and population-level structural variants with Sniffles2
Patient enrollment The 31 individuals (proband and parents) included in this study were enrolled into research protocols approved by the institutional review board (IRB) at Baylor College of Medicine and the Pacific Northwest Research Institute (H-29697 and H-47127 and WIRB 20202158). Sniffles2 methodology Sniffles2: germline calling An overview of the steps involved in the Sniffles2…
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A small-molecule activation mechanism that directly opens the KCNQ2 channel
Alsaloum, M., Higerd, G. P., Effraim, P. R. & Waxman, S. G. Status of peripheral sodium channel blockers for non-addictive pain treatment. Nat. Rev. Neurol. 16, 689–705 (2020). Article CAS PubMed Google Scholar Santos, R. et al. A comprehensive map of molecular drug targets. Nat. Rev. Drug Discov. 16, 19–34 (2017). Article CAS PubMed Google…
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Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation
Reagents and antibodies Chemical and reagents: ATP (Cat# A6559), lactate (Cat# L6661), methy-lactate (Cat# 230340), β-alanine (Cat# 146064), L-alanine (Cat# A7627), DAPI (Cat# D9542), MG132 (Cat# M8699), PPi (Cat# 433314), NAM (Cat# 72340), 13C-palmitate (Cat# 605573) and cell counting kit-8 (Cat# 96992) were purchased from Sigma-Aldrich. Oligomycin A (Cat# S1478), FCCP (Cat# S8276), α-CHCA (Cat#…
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Combined small-molecule treatment accelerates maturation of human pluripotent stem cell-derived neurons
Experimental details are summarized in Supplementary Table 2. Cell culture Human PSCs PSCs, both embryonic and induced, were maintained in Essential 8 medium (Thermo Fisher Scientific) on vitronectin-coated plates as previously described64. All stem cell work was conducted according to protocols approved by the Tri-Institutional Stem Cell Initiative Embryonic Stem Cell Research Oversight Committee (Tri-SCI…
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Spatial structure, chemotaxis and quorum sensing shape bacterial biomass accumulation in complex porous media
Fenchel, T. Microbial Behavior in a Heterogeneous World. Science 296, 1068–1071 (2002). Article ADS CAS PubMed Google Scholar Donaldson, G. P., Lee, S. M. & Mazmanian, S. K. Gut biogeography of the bacterial microbiota. Nat. Rev. Microbiol 14, 20–32 (2016). Article CAS PubMed Google Scholar Nunan, N., Schmidt, H. & Raynaud, X. The ecology of…
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Transport and inhibition mechanism for VMAT2-mediated synaptic vesicle loading of monoamines
Structural determination assisted by ALFA-tag/nanobody Human VMAT2 (55 kDa) contains a membrane domain with 12 transmembrane helices (TMs) and lacks discernable extramembrane domains (Supplementary information, Fig. S1a), posing a significant challenge for structural characterization by cryo-EM. For similarly small and featureless proteins, various approaches have been employed to increase the effective size, including specific nanobodies or antibody…
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Epigenetic checkpoints regulate the fate and function of CAR-T cells
Epigenetic checkpoints regulate the fate and function of CAR-T cells Continue Reading News Source: www.nature.com
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Lactylation constrains OXPHOS under hypoxia
Cells sense and adapt to oxygen availability. A new study published in Cell Research reports that hypoxia-induced mitochondrial protein lactylation halts oxidative phosphorylation, underscoring the swift cellular response to low oxygen. Hypoxia impacts diverse physiological processes, including cellular metabolism.1 Cells respond to oxygen deficiency by suppressing mitochondrial oxidative phosphorylation (OXPHOS) to mitigate the generation of…
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Author Correction: The self-association equilibrium of DNAJA2 regulates its interaction with unfolded substrate proteins and with Hsc70
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.…
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Integrated whole transcriptome profiling revealed a convoluted circular RNA-based competing endogenous RNAs regulatory network in colorectal cancer
In recent decades, considerable advancements in technology and clinical trials have significantly improved the detection, staging, and treatment of CRC. However, despite these developments, CRC remains one of the leading causes of cancer-related deaths worldwide24,25. One of the significant reasons for its high mortality rate and poor overall survival is late detection5. While CRC survival…