Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Reagents and antibodies

Chemical and reagents: ATP (Cat# A6559), lactate (Cat# L6661), methy-lactate (Cat# 230340), β-alanine (Cat# 146064), L-alanine (Cat# A7627), DAPI (Cat# D9542), MG132 (Cat# M8699), PPi (Cat# 433314), NAM (Cat# 72340), ¹³C-palmitate (Cat# 605573) and cell counting kit-8 (Cat# 96992) were purchased from Sigma-Aldrich. Oligomycin A (Cat# S1478), FCCP (Cat# S8276), α-CHCA (Cat# S8612) and roxadustat (Cat# S1007) were purchased from Selleck. FX-11 (Cat# HY-16214) was purchased from MedChemExpress. Lambda protein phosphatase (Cat# P0753) was purchased from New England…