Combined small-molecule treatment accelerates maturation of human pluripotent stem cell-derived neurons

Experimental details are summarized in Supplementary Table 2.

Cell culture

Human PSCs

PSCs, both embryonic and induced, were maintained in Essential 8 medium (Thermo Fisher Scientific) on vitronectin-coated plates as previously described⁶⁴. All stem cell work was conducted according to protocols approved by the Tri-Institutional Stem Cell Initiative Embryonic Stem Cell Research Oversight Committee (Tri-SCI ESCRO).

Human PSC-derived excitatory cortical neurons

Cortical neurons were generated using a protocol based on the previously described dual-SMAD inhibition paradigm⁶⁵. Briefly, hESCs were…