A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood

Borrelia burgdorferi proteins and antibodies

Recombinant OspA, OspC, and VlsE of B. burgdorferi were overexpressed in pET9a/c (OspA/OspC) and pET28a (VlsE) vectors and the proteins were purified. Briefly, IPTG-induced Escherichia coli transformed with the gene of interest were grown at 37 °C, 200 rpm, in TBY (tryptone broth yeast) supplemented with 50 μg/mL kanamycin to OD₆₀₀ of 1. The cells were harvested by centrifugation at 9000× g for 20 min at 4 °C, washed with PBS, weighed and the pellets were resuspended with BugBuster Master Mix…