Lantern-shaped flexible RNA origami for Smad4 mRNA delivery and growth suppression of colorectal cancer

Principle of the lantern-shaped flexible origami for Smad4 mRNA delivery

As shown in Fig. 1a, Smad4 mRNA was synthesized through the transcription method (IVT) method and then capped with an m7G cap at its 5’-terminal and tailed with a poly (A) tail at its 3’-terminal, which would contribute to maintaining the stability of mRNA and promoting its translation ability. Liner RNA staples with 5’-terminal phosphorylation were self-linked into circular staple RNAs (CS-RNAs) through T4 RNA Ligase. To realize the target delivery ability of the mRNA nano-lantern, RGD (Arg-Gly-Asp), a widely…