Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system

Animals

Mice were purchased from the Jackson Lab with genotypes including C57BL/6 (JAX # 000664), Thy1-EGFP-M (JAX# 007788), Thy1-YFP-16 (JAX #003709), Ai14 (JAX# 007908), Ai 140 (JAX# 030220), Shh-Cre^ERT2 (JAX# 005623) and Gli1-cre^ERT2 (JAX# 007913). All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research.

For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/mL. The solution was kept at –20 °C and delivered via intraperitoneal injection or oral…