Oral nanotherapeutic formulation of insulin with reduced episodes of hypoglycaemia

Materials

Digital heating mantle (DMS631, Adelab Scientific), silver diethyldithiocarbamate (D93503, Merck), 1-dodecanethiol (471364, Merck), cyclohexane (227048, Merck), ethanol (EtOH; 02851, Merck; 1.00983 Supelco (CSIRO)), acetone (179124, Merck; 1.00014 Supelco (CSIRO)), N₂ gas (032, BOC), Ar gas (062, BOC), carbogen gas (555, BOC), 3-MPA (M5801, Merck), chitosan (C3646, Merck), glucose (G8270, Merck; 1.00983 Supelco (CSIRO)), glacial acetic acid (A6283, Merck; 1011744 VWR Chemicals (CSIRO)), phosphate-buffered saline (P4417, Merck), EDC (E6383, Merck; E7750, Merck (CSIRO)), NHS (56485,…