Integrative genotyping of cancer and immune phenotypes by long-read sequencing

nanoranger: long-read sequencing-based genotyping of single cell RNA profiles

We developed a versatile workflow that enables the amplification, long-read sequencing, and processing of targets of interest using the ONT platform such that a wide range of natural barcodes, including somatic and mtDNA mutations, fusion genes and isoforms can be detected (Fig. 1a). The pipeline originates from single cell cDNA libraries that are whole-transcriptome amplified “intermediate libraries”. To enrich for detection of various natural barcodes, we devised a 3-step PCR protocol for targeted…