Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems

Chemicals

Potassium chloride (KCl), H₂O₂ (30%), phosphate-buffered saline (PBS), potassium phosphate monobasic (KH₂PO₄), potassium phosphate dibasic (K₂HPO₄), potassium hexachloroiridate(III) (K₃IrCl₆, Ir), and gelatin from porcine skin (gel strength ≈ 175 g Bloom, Type A, G2625) were purchased from Millipore-Sigma. All antibiotics (ampicillin, kanamycin, and chloramphenicol) were purchased from Millipore-Sigma. Lysogeny broth (LB) and agarose were from Fisher Scientific. Fluorescent beads and Pierce® horseradish peroxidase (HRP) were purchased from Thermo Fisher Scientific. 4-arm…