DNA-bridging by an archaeal histone variant via a unique tetramerisation interface

Chromatin isolation and MNase digestion

M. jannaschii DSM 2661 cells were grown in 100 l fermenters in minimal medium containing 0.3 mM K₂HPO₄, 0.4 mM KH₂PO₄, 3.6 mM KCl, 0.4 M NaCl, 10 mM NaHCO₃, 2.5 mM CaCl₂, 38 mM MgCl₂, 22 mM NH₄Cl, 31 µM Fe(NH₄)₂(SO₄)₂, 1 mM C₆H₉NO_6, 1.2 µM MgSO₄, 0.4 mM CuSO_4, 0.3 µM MnSO₄, 36 nM FeSO₄, 36 nM CoSO₄, 3.5 nM ZnSO₄, 4 nM KAl(SO₄)₂, 16 nM H₃BO₃, 42 µM Na₂SeO₄, 0.3 nM Na₂WO₄, 11 µM NaMoO₄, 44 µM (NH₄)₂Ni(SO₄)₂ and 2 mM Na₂S supplied with H₂:CO₂ gas in a 4:1 ratio at 85 °C²⁸. In all, 0.2 g wet cells…