Osteogenic induction of asiatic acid derivatives in human periodontal ligament stem cells

Materials

The derivatives of AA, 501, 502, 503, and 506 were synthesised and derivatised from AA and Asiaticoside. The chemical structures of AA and its derivatives, and the ¹H-NMR, are presented in Fig. 1 and Supplementary data, Figures S1–S4. All chemicals were of analytical grade and purchased from Sigma-Aldrich (Burlington, MA, USA) or Thermo Fisher Scientific (Waltham, MA, USA) unless otherwise stated.

Synthesis of Asiatic acid derivatives

501 compound

AA (5 g, 10.23 mmol) was added to a 100 mL round bottom flask, followed by the addition of pyridine (4.0 g, 51.2 mmol), acetic…