Two-photon nanoprobes based on bioorganic nanoarchitectonics with a photo-oxidation enhanced emission mechanism

TPA NPs preparation and characterization

Supramolecular self-assembly has been extensively used as a method to assemble NPs with novel optical properties^35,36. In this regard, amino acid derivatives are versatile building blocks due to their ease of synthesis and wide variety of physicochemical features, and have thus attracted increasing attention for the design of these NPs³⁷. In this study, the histidine (His) derivative Z-His-Obzl (ZHO) (Supplementary Fig. 1), was chosen as the template amino acid derivative to induce NIR cyanine dye ICG co-assembly due to its amphiphilic and…