Preparation of capsaicin-loaded ultrafine fiber film and its application in the treatment of oral ulcers in rats

Materials

Poly-l-lactic acid (PLLA, Mv = 40,000, Zhejiang Hisun Biomaterials Co., Ltd); gelatin (Gel, porcine skin, type A, Sigma-Aldrich), lipopolysaccharide (LPS, Sigma-Aldrich); PBS buffer (1×, pH 7.2–7.4, Solarbio), Tween 80 (Solarbio), RPMI 1640 medium (Solarbio), penicillin (Solarbio), trypsin–EDTA mixture (Solarbio); phenol (Aladdin), fluorescein isothiocyanate isomer I (90%, Aladdin); capsaicin (Cap, HPLC ≥ 98%, Chengdu Desite Biotechnology Co., Ltd.); 2,2,2-trifluoroethanol (TFE, 99.8%, Shanghai Acmec Biochemical Co., Ltd); Zoletil 50 (Virbac, China); Xylazine…