Category: 5. Biology
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SL-scan identifies synthetic lethal interactions in cancer using metabolic networks
Datasets The gene expression data, mutation data, CRISPR, and drug perturbation data sets used in this study were obtained from the Depmap project https://depmap.org/portal/download/all/. The gene expression data set consists of the log2 transformed transcript per million (TPM) values of 19,221 protein-coding genes from 1406 cell lines across 33 cancer types. We obtained the mutation…
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Comparisons among barley–pea mixed crop combinations in a replacement design as related to N fertilization and soil variation
General information Two field trials were carried out in 2017 and 2018 at the Experimental Farm of the Università Politecnica delle Marche (UNIVPM), Ancona, central Italy. The experimental area was in the lower portion of a hilly slope at an altitude between 46 and 48 m, with a S-SE exposure and gentle inclination (< 5%). Here, soils…
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Correction: α-Catenin acetylation is essential for its stability and blocks its tumor suppressor effects in breast cancer through Yap1
Correction: α-Catenin acetylation is essential for its stability and blocks its tumor suppressor effects in breast cancer through Yap1 Continue Reading News Source: www.nature.com
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A viral RNA-dependent RNA polymerase inhibitor VV116 broadly inhibits human coronaviruses and has synergistic potency with 3CLpro inhibitor nirmatrelvir
Cell lines and viruses African green monkey kidney Vero E6 cells (ATCC-1586), RD, Huh-7, HEK293T-hACE2-TMPRSS2 were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin antibiotics. MRC5 was maintained in Minimum Essential Medium (MEM) with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin antibiotics. Cells were kept at…
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Identification of transcriptional level variations in microRNA-221 and microRNA-222 as alternate players in the thyroid cancer tumor microenvironment
Heidari, Z., Mohammadpour-Gharehbagh, A., Eskandari, M., Harati-Sadegh, M. & Salimi, S. Genetic polymorphisms of miRNA let7a-2 and pri-mir-34b/c are associated with an increased risk of papillary thyroid carcinoma and clinical/pathological features. J. Cell. Biochem. 120(5), 8640–8647 (2019). Article CAS PubMed Google Scholar Torre, L. A., Siegel, R. L., Ward, E. M. & Jemal, A. Global…
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Author Correction: Two point mutations in protocadherin-1 disrupt hantavirus recognition and afford protection against lethal infection
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.…
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YAP silencing by RB1 mutation is essential for small-cell lung cancer metastasis
YAP silencing in SCLC is obligatory for tumor metastasis YAP is a transcription co-activator in the Hippo signaling pathway and is often elevated in cancer. Through pan-cancer bioinformatics analysis, we found that YAP expression is strongly downregulated in RB1 mutant SCLC cell lines, but not non-small cell lung cancer cell lines (NSCLC) (Supplementary Fig. S1a, b).…
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Microbial growth under drought is confined to distinct taxa and modified by potential future climate conditions
Cook, B. I., Mankin, J. S. & Anchukaitis, K. J. Climate change and drought: from past to future. Curr. Clim. Change Rep. 4, 164–179 (2018). Google Scholar Senf, C., Buras, A., Zang, C. S., Rammig, A. & Seidl, R. Excess forest mortality is consistently linked to drought across Europe. Nat. Commun. 11, 6200 (2020). ADS …
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DNA-bridging by an archaeal histone variant via a unique tetramerisation interface
Chromatin isolation and MNase digestion M. jannaschii DSM 2661 cells were grown in 100 l fermenters in minimal medium containing 0.3 mM K2HPO4, 0.4 mM KH2PO4, 3.6 mM KCl, 0.4 M NaCl, 10 mM NaHCO3, 2.5 mM CaCl2, 38 mM MgCl2, 22 mM NH4Cl, 31 µM Fe(NH4)2(SO4)2, 1 mM C6H9NO6, 1.2 µM MgSO4, 0.4 mM CuSO4, 0.3 µM MnSO4, 36 nM FeSO4, 36 nM CoSO4, 3.5 nM ZnSO4, 4 nM KAl(SO4)2, 16 nM H3BO3,…
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Structural basis of hydroxycarboxylic acid receptor signaling mechanisms through ligand binding
Cryo-EM structures of HCA2 and HCA3–Gi complexes with different agonists We generated HCA2 and HCA3 constructs with thermostabilized apocytochrome b562RIL (bRIL) conjugated at the N-terminus of the receptors and, for HCA2, fused the receptor to the large NanoBiT subunit (LgBiT) to stabilize the complex (NanoBiT tethering strategy20,21). These modifications had little effect on the pharmacologic…