Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase

Nuclear shape fluctuations vary with cell cycle progression

We first tested whether our cells showed a similar cell-cycle dependency of the NE flickering as observed by Chu et al. To do so, we used a GFP-tagged version of the Emerin protein to mark the NE and minimize the adverse effects of labeling on nuclear behavior, since it is well known that Lamin over-expression can directly impact mechanical properties of the nucleus²⁰. Analyzing the cell cycle duration of the HeLa cells by performing time-lapse videos (see Supplementary Information) showed that on average, in our growth conditions,…