A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells

The serine/threonine protein kinase Rim15 is required for biotrophic growth, maintaining biotrophic interfacial membrane integrity, and plant defense suppression

We disrupted the M. oryzae RIM15 coding region (MGG_00345)20 in our WT Guy11 isolate using homologous gene recombination to replace the first 1 kb of the RIM15 coding sequence with the ILV2 gene conferring sulphonylurea resistance14,21. More than ten Δrim15-carrying mutant strains were identified by PCR and two deletants, Δrim15 #7 and #9, were initially characterized and found to be indistinguishable (Fig. 1a,b and…

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