OrganoidChip facilitates hydrogel-free immobilization for fast and blur-free imaging of organoids

Undesired organoid motion and image blurring in conventional culture formats

Conventional methods for imaging organoids prove challenging because of the random locations of organoids within the wells of a multiwell plate and their proclivity towards movement during imaging. First, natural convection or fast stage movements can cause bulk media flow, potentially resulting in an organoid drifting within or even out of the FOV. Organoid drifting can result in the capturing of blurred images, while additionally the organoid’s focal plane is constantly changing during its motion in the culture…