Aging-related aneuploidy is associated with mitochondrial imbalance and failure of spindle assembly

Oocyte collection and culture

Female mice of CD1 background were obtained from Vital River Laboratory Animal Technology Co. LTD (Beijing, China), and eat and drink freely in a 23 °C temperature-controlled room with a 12 h light/dark cycle. All experimental protocols were approved by the Experimental Animal Ethics Committee of Qingdao Agricultural University. Pregnant mare serum gonadotropin (PMSG) was injected firstly, 6 weeks, and 12 months old CD1 female mice were euthanized after 48 h and subsequently ovaries were collected in M2 medium supplemented with 2.5 μM milrinone. And…