A new mechanism for crossing the blood–brain barrier
CA-IV is required for the CNS potency of Car4-dependent AAV. (A) Immunostaining for CA-IV in the brains of WT/WT and KO/KO Car4 mice. Magnified regions from WT/WT demonstrate endothelial expression across diverse brain regions. (B) AAV-PHP.eB, 9P31, and 9P36 packaging mNeonGreen under the control of the ubiquitous CAG promoter were intravenously administered to WT/WT Car4 mice at a dose of 3 × 1011 v.g. per animal (n = 3 per condition). Three weeks after administration, transgene expression was assayed by…
