Development of nanoparticles derived from corn as mass producible bionanoparticles with anticancer activity


Fetal bovine serum (FBS) was obtained from Biosera (East Sussex, UK); nonessential amino acid solution (NEAA), CPZ, sodium phosphotungstate, and 4% PFA were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). PC, cholesterol, and PE were kindly provided by Nippon Fine Chemical Co., Ltd. (Tokyo, Japan). Chloroform, methanol, penicillin–streptomycin-L-glutamine solution (× 100) (PSG), and PI were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). MβCD was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). EIPA was obtained from R&D Systems, Inc. (Minneapolis, MN, USA). Filipin III was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Alum was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco’s modified…

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