Understanding how cohesin makes DNA loops in the human genome and its role in Cornelia de Lange syndrome
NIPBL KD affects cohesin-STAG1 and cohesin-STAG2 in opposite ways. a Asynchronously growing HeLa cells mock transfected (control) or transfected with siRNA against NIPBL (NIPBL KD) were analyzed by flow cytometry 72 h later. Contour plots of the indicated proteins in control (gray plots) and NIPBL KD cells (colored plots) were overlapped for comparison. For each map, the cell cycle profile according to DNA content appears on top while the distribution of antibody intensities is plotted on the right. b Immunoblot…
