Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410

Bacterial strains, media and plasmids

In this study, Escherichia coli strains DB3.1 and MC1061 were used for cloning and Rv1410, nanobody, and megabody expression, M. smegmatis MC² 155 harboring pACE_C3GH_MHAS2168 was used for MHAS2168 protein expression and M. smegmatis MC² 155 ΔMSMEG3069/70 (dKO) was used for complementation studies. Mycobacteria were grown at 37 °C in liquid Middlebrook 7H9 medium containing 0.05% Tween 80 supplemented with OADC or on solid Middlebrook 7H10 medium supplemented with OADC containing 4.5 ml/l glycerin. For MHAS2168 expression, 7H9 medium supplemented…